The Bio-Rad CFX96 Touch™ Real-Time PCR Detection System features an integrated touch screen which allows users to set up runs quickly and monitor amplification traces in real time. This system can multiplex up to five different targets using as low as 10 uL reaction volume. The CFX96 Touch™ uses six filtered LEDs for fluorophore excitation and six filtered photodiodes to detect fluorescence emission, with an excitation range of 450-730 nm. The CFX96 Touch™ has a programmable thermal gradient, melt-curve function, and can detect as low as one copy of target sequence in human genomic DNA with a dynamic range of 10 orders of magnitude. The CFX Manager™ Software offers advanced data analysis tools for normalized gene expression, allelic discrimination, end-point analysis, and analysis of Bio-Rad PrimePCR™ Assays and Panels.
The Bio-Rad iCycler iQ™ Real-Time PCR Detection System can simultaneously measure up to four different fluorophores in each tube of a 96-well PCR plate. This system offers an excitation range of 400-700 nm, customizable filter wheels, programmable temperature gradient, as well as melt-curve and allelic discrimination analysis. The iQ™ optical system software v3.1 is available for RT-PCR product analysis.
Qubit® 3.0 Fluorometer
Qubit® 3.0 Fluorometer
The Qubit® 3.0 Fluorometer accurately measures dsDNA, oligos, RNA, microRNA, and protein at low concentrations, using as little as 1 uL of sample. The Qubit® 3.0 is more sensitive than UV absorbance, as it uses highly sensitive Qubit® quantitation assays containing fluorescent dyes that emit a signal only when bound to the target molecule. This specificity minimizes the effects of contaminants such as free nucleotides, intact/degraded DNA and RNA, protein, and excess salts. The Qubit® 3.0 is ideal to quantify samples used in downstream applications such as quantitative real-time PCR or next-gen sequencing. The Qubit® 3.0 can also be used as a mini-fluorometer to generate RFU values in the 300 - 1,000 nm range by selecting either the blue LED excitation source with emission in the green and far-red channels, or the red LED excitation source with emission the far-red channel.
Typhoon 9410 Variable Mode Imager
The GE Typhoon 9410 Variable Mode Imager can scan and detect nucleic acids and proteins labeled with single or multicolor fluorescent, phosphorescent, or chemiluminescent probes. This platform can scan gels, blots, dishes, plates, microplates, and glass slide microarrays. Available excitation wavelengths are 457/488/532/633 nm and available emission filters range from 500 to 685 nm, which allow for collection of specific and multiple signals. The sensitive optics of the imager enables direct chemiluminescent imaging without intermediate exposures to films or screens. This platform can produce storage phosphor imaging of radioisotope sources. The UNLV Genomics Core Facility offers ImageQuant 5.2, FluorSep 2.2, Fragment Analysis 1.1 and IQ tools 2.2 software for image analysis.
OmniGrid 100 Microarrayer
The DigiLab Genomic Solutions® OmniGrid 100 Microarrayer is a high capacity multi-axis microarrayer that prints DNA samples onto solid substrates. This instrument is fully automated and is capable of handling of up to 72 input plates and holds 100 standard 1" x 3" glass slides. This instrument possesses a 48-pin head that can print in excess of 100,000 features per slide with a resolution of 2.5 µm on the X and Y-axis and 1.25 µm on the Z-axis. The samples are spotted onto the slides in patterns of rows and columns known as sample groups that can be adjusted according to personal preference.
GenePix® 4000B Scanner
The GenePix® 4000B Scanner can be used to scan DNA and protein microarrays as well as tissue and cell arrays. This scanner has dual lasers that can acquire data at wavelengths of 532 nm (Cy 3) and 635 nm (Cy 5) simultaneously. This instrument scans standard 1" x 3" glass slides and produces images with a resolution range of 5 µm to 100 µm. GenePix® Pro software is available for microarray image acquisition and data analysis, which uses industry standard GAL and GPR file formats.
The Nanodrop 1000 is a full spectrum spectrophotometer (UV and visible spectrum, 220-750 nm) that can measure the absorbance of DNA, RNA, protein and dyes using as little as 1 µl sample volume without the use of cuvettes or capillaries. This instrument is routinely used to quantify and check the purity of RNA and DNA before proceeding with PCR or microarray assays.
SpectraMax Plus Spectrophotometer/Platereader
The SpectraMax Plus384 is a full spectrum spectrophotometer (190-1000 nm) which contains both a cuvette port and a microplate drawer. Any standard cuvette, 12 x 75 mm test tube, 96 or 384-well microplate can be used to obtain absorbance measurements.
Hyb4 Microarray Hybridization System
Hyb4 Microarray Hybridization System
The Hyb4 is a microarray hybridization system that functions to automate thermal and wash cycles for hybridization of microarrays spotted on standard glass slides.
Avanti J-E Centrifuge
The Beckman Coulter Avanti J-E centrifuge is a refrigerated floor model preparative centrifuge. The micro-processor controlled Avanti J-E can be programmed to run at temperatures between 40°C and -10°C. Available rotors include the JA-25.50 rotor with a maximum speed of 21,000 rpm, and the JA-10 rotor with a maximum speed of 10,000 rpm.
The UltraPro 5000 Cryostat is a temperature controlled system used to prepare frozen sections of biological tissues for microscopic examination. This instrument includes a 5040 rotary retracting microtome and allows for tissue orientation in the x and y plane, as well as antiroll plate angle adjustment.
Eppendorf Mastercycler Gradient
The Eppendorf Mastercycler gradient is a thermal cycler designed for the amplification of nucleic acids. This thermal cycler possesses a programmable heated lid and gradient function, which allows the entire block temperature to be varied within a 20°C range. Peltier elements control rapid heating speeds up to 3°C per second and cooling speeds up to 2°C per second in a temperature range of 4°C to 99°C. The block will accommodate one 96-well PCR plate, 96 x 0.2 ml PCR tubes, or 77 x 0.5 ml PCR tubes.
Eppendorf Mastercycler Personal
The Eppendorf Mastercycler personal is a thermal cycler designed for the amplification of nucleic acids. This thermal cycler possesses a programmable heated lid and sample block which can accommodate 25 x 0.2 ml PCR tubes, 16 x 0.5 ml PCR tubes or one 25-well PCR plate. Peltier elements control rapid heating speeds up to 3°C per second and cooling speeds up to 2°C per second in a temperature range of 4°C to 99°C.
Applied Biosystems GeneAmp® PCR 9700
The GeneAmp® PCR System 9700 is designed for the amplification of nucleic acids. The sample block can accommodate either 96 x 0.2 ml PCR tubes or one 96-well PCR plate. The gold-plated silver block delivers rapid heating and cooling speeds up to 5°C per second, decreasing cycle times for volumes up to 100 µl.
DNASTAR Lasergene v8.0 Software
DNASTAR Lasergene version 8.0 is a comprehensive software pack used for the analysis of DNA and protein sequences. This software consists of an integrated suite of seven modules that facilitate sequence visualization and editing, primer design, virtual cloning, sequence assembly and alignment, Next-Gen sequence analysis and SNP discovery, gene finding, protein structure analysis and prediction and contains a utility for importing unusual file types.
The QIAGEN TissueLyser provides efficient disruption of tissues and cells through high speed shaking and grinding effects of stainless steel beads on biological samples. Up to 48 samples can be disrupted in 2 to 4 minutes, which can then be used to extract RNA, DNA and protein for downstream molecular applications.
CentriVap Refrigerated Concentrator
The Labconco CentriVap refrigerated concentrator serves to rapidly concentrate small volumes of heat sensitive biological samples, such as RNA, DNA and protein. The CentriVap uses centrifugal force (max rotor speed of 1,725 rpm) coupled with vacuum and a temperature controlled environment ranging from -4°C to 100°C to accelerate the evaporation of up to 132 sample volumes of 1 ml or less.
Beckman Coulter Optima L-90K Ultracentrifuge
The Beckman Coulter Optima L-90K is a floor model preparative ultracentrifuge that is able to generate 694,000 x g at speeds up to 90,000 rpm. Available rotors include the SW 41 Ti rotor, with a maximum speed of 41,000 rpm, the SW 55 Ti rotor, with a maximum speed of 55,000 rpm and the Type 50.2 Ti rotor, with a maximum speed of 50,000 rpm, and the Type 90 Ti rotor, with a maximum speed of 90,000 rpm.
Eppendorf Centrifuge 5810 R
The Eppendorf 5810 R is a refrigerated bench top centrifuge. Available rotors include the FA-45-30-11 rotor, which will accommodate 1.5 ml or 2.0 ml microcentrifuge tubes with a maximum speed of 14,000 rpm. The A-4-62 rotor is also available, which will accommodate 15 ml and 50 ml tubes, as well as 96 well plates with a maximum speed of 4,000 rpm.
Eppendorf Centrifuge 5424
The Eppendorf 5424 is a non-refrigerated bench top centrifuge that is able to generate speeds up to 15,000 rpm. Two standard aerosol tight rotors are available that will accommodate up to 24 x 1.5 ml or 2.0 ml microcentrifuge tubes, or 8 x 0.2 ml PCR strip tubes.
CL-1000 UV Crosslinker
The UVP CL-1000 Ultraviolet Crosslinker produces shortwave ultraviolet radiation at 254 nm which can be utilized to covalently bind nucleic acids to nitrocellulose, nylon, and nylon-reinforced membranes for Northern, Southern, slot or dot blotting applications. This system can also be used to nick Ethidium Bromide stained DNA in agarose gels for CHEF assays, control the extent of restriction enzyme digestions, detect recA mutations, and sterilize small surfaces by killing bacteria, viruses, bacteriophages and small organisms.
DCode Universal Mutation Detection System
The Bio-Rad DCode Universal Mutation Detection System can be used to screen for DNA polymorphisms. This system provides a controlled temperature environment ranging from 5-70°C and is capable of running up to 64 samples in 2 hours. This system can detect single-base changes through single-stranded conformation polymorphism, denaturing gradient gel electrophoresis, temporal temperature gradient electrophoresis, and constant denaturing gel electrophoresis techniques.
The high capacity VWR hybridization oven functions to meet hybridization needs for Northern, Southern and Western blotting. This oven holds 100, 150, 225 and 300 mm length bottles and can also be adapted as a platform rocker to hold plastic containers or heat sealed bags. This oven is equipped with an adjustable speed carousel and produces three-dimensional motion to provide excellent membrane coverage.
The VWR incubator model 1525 is a general purpose incubator. This incubator is set at 37°C and is without gas or humidity. The temperature range of this unit is 5°C to 70°C.
SDS PAGE and Western Blot Equipment
The UNLV Genomics Core Facility offers all the hardware necessary to run SDS PAGE and Western blots, including single and multi-gel casting systems, rigs, power packs, a gel dryer and a Bio-Rad Bio-Dot SF apparatus.
2D Gel Electrophoresis Equipment
The UNLV Genomics Core Facility offers all the hardware necessary to run 2D gel electrophoresis.
DNA Agarose Gel Equipment
The UNLV Genomics Core Facility offers all of the hardware needed to run DNA agarose gels, including gel rigs, combs, power packs and a UV illuminator.
BioRad Gel Dryer Model 583
The BioRad Gel Dryer Model 583 is designed to dry PAGE, gradient and sequencing gels. The gel dryer contains three preset microprocessor-controlled heating cycles combined with vacuum which provide optimal drying conditions for different gel types. Manual programming allows the user to select any drying temperature between 50°C and 90°C. Gels can be dried on either filter paper or cellophane membranes.
Techne TC-5000 Gradient Thermal Cycler
The Techne TC-5000 is a touch screen gradient thermal cycler designed for the amplification of nucleic acids. This thermal cycler has a programmable heated lid and gradient function, which allows the entire block temperature to be varied within a 30°C range. The TC-5000 has a typical heating rate of 3°C per second and cooling rate of 1.3°C per second in a temperature range of 4°C to 99°C. The block will accommodate one 96-well PCR plate or 96 x 0.2 ml PCR tubes.
Lab-Line Environmental Chamber
The Lab-Line Environette Environmental Chamber can reach temperatures up to 60 degrees Celsius. The interior dimensions are approximately 35 inches wide by 24 inches deep by 66 inches high. A power outlet is available inside of the chamber.
The UNLV Genomics Core Facility offers a variety of other equipment to support one's research needs, including balances, pH meter, shakers, rockers, thermomixers, a Nanopure water system and a cold room.